John M. Pascal, PhD

Contact Dr. Pascal

233 South 10th Street
Suite 804
Philadelphia, PA 19107

(215) 503-4596
(215) 923-2117 fax

Research and Clinical Interests
Structural biology of DNA replication, repair and signaling mechanisms; mechanisms of genome maintenance and chromosomal metabolism; biochemical and biophysical studies of nucleic ACID enzymes; macromolecular x-ray crystallography

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Publications

Most recent Peer-reviewed Publications

1. Structural biology of the writers, readers, and erasers in mono- and poly(ADP-ribose) mediated signaling
2. Unstructured to structured transition of an intrinsically disordered protein peptide in coupling Ca2+-sensing and SK channel activation
3. PARP-1 mechanism for coupling DNA damage detection to poly(ADP-ribose) synthesis
4. Dual roles of PARP-1 promote cancer growth and progression
5. Identification of the functional binding pocket for compounds targeting small-conductance Ca 2+ -activated potassium channels
6. Structural basis for DNA damage-dependent poly(ADP-ribosyl)ation by human PARP-1
7. Structural basis for calmodulin as a dynamic calcium sensor
8. Autoregulation of kinase dephosphorylation by ATP binding to AGC protein kinases
9. Adaptation to tRNA acceptor stem structure by flexible adjustment in the catalytic domain of class I tRNA synthetases
10. Resistance of Akt kinases to dephosphorylation through ATP-dependent conformational plasticity
11. Purification of human PARP-1 and PARP-1 domains from escherichia coli for structural and biochemical analysis
12. Crystal structures of poly(ADP-ribose) polymerase-1 (PARP-1) zinc fingers bound to DNA: Structural and functional insights into DNA-dependent PARP-1 activity
13. The Zn3 domain of human poly(ADP-ribose) polymerase-1 (PARP-1) functions in both DNA-dependent poly(ADP-ribose) synthesis activity and chromatin compaction
14. The DNA binding domain of human DNA ligase I interacts with both nicked DNA and the DNA sliding clamps, PCNA and hRad9-hRad1-hHus1
15. A third zinc-binding domain of human poly(ADP-ribose) polymerase-1 coordinates DNA-dependent enzyme activation (Journal of Biological Chemistry (2008) 283, (4105-4114))
16. A third zinc-binding domain of human poly(ADP-ribose) polymerase-1 coordinates DNA-dependent enzyme activation
17. DNA and RNA ligases: structural variations and shared mechanisms
18. RNA ligase does the AMP shuffle
19. A Flexible Interface between DNA Ligase and PCNA Supports Conformational Switching and Efficient Ligation of DNA
20. Human DNA Ligases I, III, and IV-Purification and New Specific Assays for These Enzymes

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