Eleni Anni, PhD
Jefferson Alumni Hall, Suite 314A
Philadelphia, PA 19107
Most Recent Peer-reviewed Publications
- Structure of zinc-substituted cytochrome c: Nuclear magnetic resonance and optical spectroscopic studies
- Resonance Raman spectra of large pea phytochrome at ambient temperature. Difference in chromophore protonation between red- and far red-absorbing forms
- Formation and decay of cytochrome c peroxidase compound ES during aerobic reduction with dithionite
- Structural characterization of cytochrome c peroxidase by resonance Raman scattering
- The coordination and spin states of yeast cytochrome c peroxidase and their implication to peroxidase mechanism.
PhD, University of Patras, Greece - 1984
Research and Clinical Interests
The focus of the current research in Dr. Anni's laboratory is on understanding how ethanol reprograms the complex genetic and epigenetic regulatory networks operating at the onset of embryonic development in Fetal Alcohol Spectrum Disorder (FASD). The disease is caused by maternal consumption of alcohol during pregnancy, and is the leading cause of mental retardation. Using mouse embryonic stem cells to recapitulate the early stages of neurogenesis we have shown that ethanol differentially regulates the mRNA and protein expression levels of several transcription factors, including Sox2 and Nanog that govern cell fate commitment. Techniques used include high-throughput multiparametric flow cytometry and microfluidic-based RT-qPCR. Network analysis, in collaboration with Dr. Vadigepalli, has predicted altered dynamics and downstream target dysregulation that may be responsible for the FASD phenotype. The goal of these integrated experimental and computational modeling studies is to identify the critical network components affected by ethanol exposure in early embryonic development and their contribution to the FASD phenotype.