Thomas Jefferson University - Kyonggeun Yoon
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Kyonggeun Yoon
Dermatology and Cutaneous Biology
Thomas Jefferson University
Jefferson Medical College
Department of Dermatology & Cutaneous Biology
Associate Professor
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Expertise and Research Interests
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My research interests are developing new gene therapy technologies, gene repair by oligonucleotides, for potential treatment of genetic or acquired skin diseases. The epidermis is an ideal organ for testing and developing different modalities of gene therapy. The accessibility of the tissue makes it possible to administer therapeutics locally, to monitor the treatment site, and to compare with untreated site easily. The goal of this novel approach is to correct a specific mutation while maintaining the complex genomic organization important for the appropriate expression and regulation of gene. My research interests are diverse ranging from biochemical mechanism of gene repair to practical application to correct the heritable defects of patients with debilitating skin blisters.
As a director of the Cutaneous Gene Therapy Group, my laboratory serves as a training ground for more conventional gene therapies by viral vectors, adenovirus and retrovirus. Several in vivo delivery methods have also been developed in my laboratory, gene gun, topical application, and intradermal injection.
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Other Expertise
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Combining my chemistry background in nucleic acids with molecular cellular biology experiences, I have also developed several technologies in nucleic-acid-based therapeutics, antisense, ribozyme, triple-helix and SELEX for anti-inflammation and anti-viral agents.
Recently, my interests have expanded to RNA interference.
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Keywords
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Dna Repair And Recombination; Gene Targeting; Gene Therapy; Oligonucleotide; Rnai; Transcription
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Publications
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- Igoucheva, O., Alexeev, V. Pryce, M. and Yoon, K. Transcription affects formation and processing of intermediates in gene correction by oligonucleotides. Nucleic Acids Research. 31: 2659-2670, 2003
- Pierce, E. A., Liu, Q., Ma, H.C. Igoucheva, O., Diamond, S. A. and Yoon, K. Oligonucleotide-directed single base DNA alterations in mouse embryonic stem cells. Gene Therapy. 10: 24-33, 2003
- Alexeev, V. Igoucheva, O., and Yoon, K. Simultaneous targeted alteration of the tyrosinase and c-kit genes by single-stranded oligonucleotides. Gene Therapy. 9: 1667-1675, 2002
- Igoucheva, O., Alexeev, V. and Yoon, K. Nuclear extracts promote gene correction and strand pairing of oligonucleotides to the homologous plasmid. Antisense and nucleic acid drug development. 12: 235-246, 2002
- Multilineage Differentiation of Adult Human Bone Marrow Progenitor Cells Transduced with Human Papillomavirus-16 E6/E7 Genes. Calcified Tissue International. 71: 447-458, 2002
- Yoon, K. , Igoucheva, O. and Alexeev, V. Targeted gene repair-Expectations and Reality. Nature Biotechnology. 20: 1197-1198, 2002
- Lin M, Wang F, Uitto J, Yoon K. Differential expression of tissue-specific promoters by gene gun. British Journal of Dermatology. 144: 34-39, 2001
- Igoucheva, O., Alexeev, V. and Yoon, K. Targeted gene correction by small single-stranded oligonucleotides in mammalian cells. Gene Therapy. 8: 391-399, 2001
- Yang, S. Tutton, S. Pierce, E.A. and Yoon, K. Specific double-stranded RNA interference in undifferentiated mouse embryonic stem cells. Molecular and Cellular Biology. 21: 7807-7816, 2001
- Igoucheva, O. and Yoon, K. Methods for determining gene correction frequency by chimeric RNA-DNA oligonucleotide using nuclear extracts. Gene Therapy Protocols, Methods in Molecular Medicine, ed., Morgan, J.R. Humana Press. 95-108, 2001
- Alexeev V, Yoon K. Gene correction by RNA-DNA oligonucleotides. Pigment Cell Research. 13(2): 72-9, Apr 2000
- Lin MT, Pulkkinen L, Uitto J, Yoon K. The gene gun: current applications in cutaneous gene therapy. International Journal of Dermatology. 39(3): 161-70, Mar 2000
- Alexeev V, Igoucheva O, Domashenko A, Cotsarelis G, Yoon K. Localized in vivo genotypic and phenotypic correction of the albino mutation in skin by RNA-DNA oligonucleotide [see comments]. Nature Biotechnology. 18(1): 43-7, Jan 2000
- Igoucheva O, Yoon K. Improvement of RNA-DNA oligonucleotide design by using mammalian nuclear extracts. Gene Therapy and Regulation. 1: 165-177, 2000
- Lin MTS, Pulkkinen L, Uitto J, Yoon K. The gene gun: Current applications in cutaneous gene therapy. Bio-Rad Technical Note. 2552, 2000
- Igoucheva, O. and Yoon, K. Targeted single base correction by RNA-DNA oligonucleotides. Human Gene Therapy. 11: 2307-2313, 2000
- Yoon, K. and Alexeev, V. Retroviral delivery of ECM genes to cells. Methods in molecular biology , ed. Streuli, C. and Grant, M., Humana Press. 197-208, 2000
- Yoon, K. Single-base conversion of mammalian genes by an RNA-DNA oligonucleotide. Progress in gene Therapy: Basic and Clinical Frontiers, ed, Bertolotti, R. 475-512, 2000
- Igoucheva O, Peritz AE, Levy D, Yoon K. A sequence-specific gene correction by an RNA-DNA oligonucleotide in mammalian cells characterized by transfection and nuclear extract using a lacZ shuttle system. Gene Therapy. 6(12): 1960-71, Dec 1999
- Yoon K. Single-base conversion of mammalian genes by an RNA-DNA oligonucleotide. Biogenic Amines. 15: 137-167, 1999
- Yoon, K, Jendis, J. and Moelling, K. RNA and triple helices. Triple helix Forming Oligonucleotides, ed. Malvy, C. and Harel-Bellan, A., Kluwer Academic Pub. 233-242, 1999
- Santana E, Peritz AE, Iyer S, Uitto J, Yoon K. Different frequency of gene targeting events by the RNA-DNA oligonucleotide among epithelial cells. Journal of Investigative Dermatology. 111(6): 1172-7, Dec 1998
- Alexeev V, Yoon K. Stable and inheritable changes in genotype and phenotype of albino melanocytes induced by an RNA-DNA oligonucleotide [see comments]. Nature Biotechnology. 16(13): 1343-6, Dec 1998
- Cole-Strauss A, Yoon K, Xiang Y, Byrne BC, Rice MC, Gryn J, Holloman WK, Kmiec EB. Correction of the mutation responsible for sickle cell anemia by an RNA-DNA oligonucleotide [see comments]. Science. 273(5280): 1386-9, Sep 1996
- Yoon K, Cole-Strauss A, Kmiec EB. Targeted gene correction of episomal DNA in mammalian cells mediated by a chimeric RNA.DNA oligonucleotide. Proceedings of the National Academy of Sciences (USA). 93(5): 2071-6, Mar 1996
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Individual Expertise profile of
Kyonggeun Yoon, Copyright © Kyonggeun Yoon.
Last Updated
by Admin
: Thursday, December 1, 2005 9:47:39 AM
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