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Thomas Jefferson University - Gino Cingolani, PhD
Gino Cingolani, PhD

Biochemistry & Molecular Biology
Associate Professor
Mailing Address
233 South 10th Street
BLSB Room 826
Philadelphia, Pennsylvania 19107
United States
Contact Information
Phone: 215-503-4573
Fax: 215-923-2117
Gino.Cingolani@jefferson.edu
Personal Web Site
Qualifications
B.S./M.S. in Biochemistry, University of Bari, Italy, 1995

Ph.D. in Structural Biology, European Molecular Biology Laboratory, 1999

Human Frontier Science Program Postdoctoral fellow, Scripps Research Institute, 1999-2003
Expertise and Research Interests
My laboratory uses X-ray crystallography, combined with biophysical and biochemical techniques to probe the structure and function of large macromolecular machines. We are interested in a variety of biological problems, which include the mechanisms and regulation of nucleocytoplasmic transport, the delivery of viral DNA into living cells, and the structure and regulation of multi-subunit ATP synthases and disease-related dual specificity phosphatases. Our structural approach provides a unique tool to understand the function and engineer the activity of biological molecules. Visit our website (http://www.cingolanilab.org/) for more info.
Keywords
X-ray crystallography; Structural Biology; Nucleocytoplasmic Transport; Dual Specificity Phosphatase; Virus Assembly; Viral Genome Ejection/Packaging; ATP-synthase; Multisubunit ATPases
Languages
English, Italian, French, some Portuguese
Publications
  • Sankhala, S.R., Lokareddy, K.R. and Cingolani, G. (2014) Structure of Human PIR1, an Atypical Dual Specificity Phosphatase. Biochemistry. 53(5):862-71.
  • Bhardwaj, A., Olia, A.S. and Cingolani, G. (2014) Architecture of Viral Genome-Delivery Molecular Machines. Curr Opin Struct Biol. 25:1-8.
  • Bhardwaj, A., Casjens, S.R. and Cingolani, G. (2014). Exploring the atomic structure and conformational flexibility of a 320A long engineered viral fiber using X-ray crystallography. Acta Cryst. D70, 342-353.
  • Pumroy, R. and Cingolani, G. (2013) Jamming up the "beta-staple": Regulation of SIRT1 activity by its C-terminal regulatory segment (CTR). J. Mol. Biol. 10.1016/j.jmb.2013.11.013.
  • Smith F.J., Pau, V.P., Cingolani, G., and Rothberg, B.S. (2013) Structural basis of allosteric interactions among Ca2+-binding sites in a K+ channel RCK domain. Nature Commun. 4:2621.
  • Leavitt, J.C. Gogokhia, L., Gilcrease, E.B., Bhardwaj, A., Cingolani, G. and Casjens, S.R. (2013) The tip of the tail needle affects the rate of DNA delivery by bacteriophage P22. PLOS One, 8(8):e70936.
  • Lokareddy, K.R., Bhardwaj, A. and Cingolani, G. (2013) Atomic structure of dual-specificity phosphatase 26, a novel p53 phosphatase. Biochemistry, 52(5):938-48.
  • Smith, F.J., Pau, V.P., Cingolani, G. and Rothberg, B.S. (2012) Crystal Structure of a Ba2+-bound gating ring reveals elementary steps in RCK domain activation. Structure. 20(12):2038-47.
  • Roy, A., Hutcheon, L., Duncan, T.M. and Cingolani, G. (2012) Improved crystallization of Escherichia coli ATP synthase catalytic complex (F1) by introducing a phosphomimetic mutation in subunit ยต. Acta Cryst. F. 68, 1229-1233.
  • Roy, A. and Cingolani, G. (2012) Structure of P22 headful packaging nuclease. J Biol Chem. 287(33):28196-205.
  • Roy, A., Bhardwaj, A., Datta, P., Lander, G.C. and Cingolani, G. (2012) Small terminase couples viral DNA-binding to genome-packaging ATPase activity. Structure. 20(8):1403-13.
  • Pumroy, R.A., Nardozzi, J.D., Hart, D.J., Root, M.J., Cingolani, G. (2012) Nucleoporin Nup50 Stabilizes Closed Conformation of Armadillo repeat 10 in Importin a5. J Biol Chem. 287(3):2022-31.
  • Bhardwaj, A., Molineux, J.I., Casjens, S.R. and Cingolani, G. (2011) Atomic Structure of Sf6 Tail Needle Knob. J Biol Chem. 286(35):30867-77.
  • Lott K, Bhardwaj A, Sims P.J., Cingolani G. (2011) A minimal NLS in human phospholipid scramblase 4 that binds only the minor NLS-binding site of importin a1. J Biol Chem. 286(32):28160-9.
  • Cingolani, G. and Duncan, T.M. (2011) Structure of the ATP synthase catalytic complex (F1) from Escherichia coli in an auto-inhibited conformation. Nature Struc Mol Biol. 18(6):701-7.
  • Olia, A.S, Prevelige Jr., P.E., Johnson, J.E. and Cingolani, G. (2011) Three-dimensional structure of a viral genome-delivery portal vertex. Nature Struc Mol Biol. 18(5):597-603.
  • Tang, J., Lander, G., Olia, A.S, Prevelige Jr., P.E., Cingolani, G., Casjens, S., Baker, T.S. and Johnson, J.E. (2011) Peering down the barrel of a bacteriophage portal: the genome packaging and release valve in P22. Structure. 19(4):496-502.
  • Koksal, A. and Cingolani, G. (2011) Dimerization of Vaccinia virus VH1 is essential for dephosphorylation of STAT1 at tyrosine 701. J Biol Chem. 286(16):14373-82.
  • Roy, A., Bhardwaj, A., and Cingolani, G. (2011) Crystallization of the Nonameric Small Terminase Subunit of Bacteriophage P22. Acta Cryst. F. 67, 104-110.
  • Nardozzi, J., Lott, K. and Cingolani, G. (2010) Phosphorylation meets Nuclear Import: a review. Cell Communication and Signaling. 8(1):32.
  • Lott, K. and Cingolani, G. (2011) The IBB-domain as a master regulator of nucleocytoplasmic transport. Biochim Biophys Acta. 1813(9):1578-92.
  • Nardozzi, J., Wenta, N., Yasuhara, N., Vinkemeier, U. and Cingolani, G. (2010) Molecular basis for the recognition of phosphorylated STAT1 by importin a5. J. Mol. Biol. 402(1):83-100.
  • Bhardwaj, A. and Cingolani, G. (2010) Conformational selection in the recognition of snurportin IBB-domain by importin b. Biochemistry 49(24):5042-7.
  • Lott, K., Bhardwaj, A., Mitrousis, G., Pante, N., and Cingolani, G. (2010) The importin beta binding domain modulates avidity of importin b for the nuclear pore complex. J. Biol. Chem. 285(18):13769-80.
  • Bhardwaj, A., Walker-Kopp, N., Casjens, S.R., and Cingolani, G. (2009) An evolutionarily conserved family of virion tail needles related to bacteriophage P22 gp26: correlation between structural stability and length of the a-helical trimeric coiled-coil. J. Mol. Biol. 391(1):227-45.
  • Mitrea, D.M., Butler, J.S., Mitrousis, G., Cingolani, G. and Loh, S. N. (2009). Structural and Thermodynamic Analysis of Conformationally-Strained Circular Permutants of Barnase. Biochemistry. 48(15):3497-507.
  • Koksal, A., Nardozzi, J., and Cingolani, G. (2009) Dimeric quaternary structure of the prototypical dual specificity phosphatase VH1. J. Biol. Chem. 284(15):10129-37.
  • Olia, S.A., Casjens, S., and Cingolani, G. (2009) Structural Plasticity of the phage P22 tail-needle gp26 probed with Xenon Gas. Protein Science. 18(3):537-548.
  • Olia, S.A., and Cingolani, G. (2008) A shifty stop for a hairy tail. Mol. Microbiology, 70(3):549-53.
  • Bhardwaj, A., Walker-Kopp N, Wilkens, S., and Cingolani, G. (2008) Foldon-guided self-assembly of ultra stable protein fibers. Protein Science 17(9):1475-85.
  • Lorenzen, K., Olia, S.A., Uetrecht, C., Cingolani, G., and Heck, A.J.R. (2008). Determination of stoichiometry and conformational changes in the first step of the P22 tail assembly. J. Mol. Biol. 379(2):385-96.
  • Mitrousis, G., Olia, S.A., Walker and Cingolani, G. (2008). Molecular basis for the recognition of snurportin 1 by importin b. J. Biol. Chem. 283(12):7877-84.
  • Zheng, H., Olia, S.A., Hankins, M., Andrews, S., Cingolani, G., and Gonen, T, (2008). A conformational switch in bacteriophage P22 portal protein primes genome injection. Molecular Cell. 29(3), 376-383.
  • Olia, S.A., Casjens, S. and Cingolani, G. (2007) Structure of Phage P22 Cell Envelope Penetrating Needle. Nature Struc Mol Biol. 14(12), 1221-1226.
  • Olia, S.A., Bhardwaj, A., Joss, L., Casjens, S., and Cingolani, G. (2007) The role of gene 10 protein in the hierarchical assembly of the bacteriophage P22 portal vertex structure. Biochemistry. 46(30), 8776-8784.
  • Bhardwaj, A., Olia, S.A., Walker-Kopp N, and Cingolani, G. (2007) Domain organization and polarity of tail needle gp26 in the portal vertex structure of bacteriophage P22. J. Mol. Biol. 371(2), 374-87.
  • Olia, A., Al-Bassam, J., Winn-Stapley, D.A., Joss, L., Casjens, S., and Cingolani, G. (2006) Binding induced stabilization and oligomerization of portal closure factor gp4 in the assembly pathway of phage P22 tail. J. Mol. Biol. 363, 558-576.
  • Cingolani, G., Andrews, D., and Casjens, S. (2006) Crystallogenesis of bacteriophage P22 tail accessory factor gp26 at acidic and neutral pH. Acta Cryst. F 62, 477-482.
  • Taylor, D.J., Speir, J.A., Reddy, V., Cingolani, G., Pringle, F.M., Ball, L.A. and Johnson, J.E. (2006) Preliminary X-ray characterization of authentic providence virus and attempts to express its coat protein gene in recombinant baculovirus. Arch Virol. 151(1):155-65.
  • Andrews D, Butler JS, Al-Bassam J, Joss L, Winn-Stapley DA, Casjens S, and Cingolani, G. (2005) Bacteriophage P22 Tail Accessory Factor GP26 Is a Long Triple-stranded Coiled-coil. J. Biol. Chem. 280(7): 5929-33.
  • Chen MH, Ben-Efraim I, Mitrousis G, Walker-Kopp N, Sims PJ, and Cingolani, G. (2005) Phospholipid scramblase 1 (PLSCR1) contains a non-classical nuclear localization signal with unique binding site in importin a. J. Biol. Chem. 280(11):10599-606.
  • Tang L., Marion W., Cingolani, G., Prevelige P.E. Jr., and Johnson J.E. (2005) Three-dimensional structure of the bacteriophage P22 tail machine EMBO J. 24(12):2087-95.
  • Wodrich, H., Guan, T., Cingolani, G., von Seggern, D., Nemerow, G., and Gerace, L. (2003). Switch from capsid protein import to adenovirus assembly by cleavage of nuclear transport signals. EMBO J. 22 (23): 6150-6162.
  • Bednenko, J., Cingolani, G., and Gerace, L. (2003). Importin beta contains a COOH-terminal nucleoporin binding region important for nuclear import. J. Cell Biol. 162(3): 391-401.
  • Koerner, C., Guan, T., Gerace, L. and Cingolani, G. (2003). Synergistic effects of silent and hot spot mutations in importin b reveals a dynamic mechanism for recognition of a nuclear localization signal. J. Biol. Chem. 278 (18): 16216-16221.
  • Cingolani, G., and Gerace, L. (2003). Molecular basis of nucleocytoplasmic transport. Handbook of Cell Signaling, Academic Press, Vol. 3(330): 419-429.
  • Bednenko, J., Cingolani, G., and Gerace, L. (2003). Nucleocytoplasmic transport: navigating the channel (NPC). Traffic, 4(3): 27-135.
  • Cingolani, G., Bednenko, J., Gillespie, M., and Gerace, L. (2002). Molecular basis for the recognition of a non-classical nuclear localization signal (NLS) by importin b. Molecular Cell, 10: 1345-1353.
  • Cingolani, G., Moore, S., Privilege, P., and Johnson, J. (2002). Preliminary crystallographic analysis of the bacteriophage P22 portal protein. J. Struc. Biology, 139: 46-54.
  • Cingolani, G., Lashuel, H., Gerace, L., and Mueller, C.W. (2000). Nuclear import factors importin a and importin b undergo mutually induced conformational changes upon association. FEBS Lett., 484(3): 291-298.
  • Chook, Y.M, Cingolani, G., Conti, E., Stewart, M., Vetter, I., and Wittinghofer, A. (1999). Pictures in cell biology. Structures of nuclear-transport components. Trends Cell Biol., 9(8): 310-311
  • Cingolani, G., Petosa, C., Weis, K., and Mueller, C.W. (1999). Structure of importin b bound to the IBB domain of importin a. Nature, 399(6733): 221-229.
  • Cingolani, G., Capaccio, L., D'Elia, D., and Gadaleta, G. (1997). In organello footprinting analysis of rat mitochondrial DNA: protein interaction upstream of the Ori-L. Biochem Biophys Res Commun, 231(3): 856-860.

Individual Expertise profile of Gino Cingolani, PhD, Copyright © Gino Cingolani, PhD.
Last Updated by Gino Cingolani, PhD : Sunday, September 22, 2013 9:46:24 PM



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