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Thomas Jefferson University - Shiu-Ying Ho, Ph.D.
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Shiu-Ying Ho, Ph.D.
Biochemistry and Molecular Biology
Thomas Jefferson University
Jefferson Medical College
Department of Biochemistry & Molecular Biology
Research Assisitant Professor
Director, Zebrafish Facility,
Kimmel Cancer Center
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Mailing Address
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Rm 484, 1020 Locust Street
Philadelphia, Pennsylvania 19107
United States
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Qualifications
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Ph.D. Rutgers University, Nutritional Sciences, 2000.
American Heart Association Postdoctoral Fellowship, 2001-2004
NIH individual NRSA Postdoctoral Fellowship, 2004-2005
American Heart Association SDG Award, 2007-present
Member, American Association for the Advancement of Science
Member, American Society for Biochemistry and Molecular Biology
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Expertise and Research Interests
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Research in my laboratory focuses on how lipids are absorbed, transported, and metabolized. The ability to regulate lipid metabolism has profound clinical implications in humans because a variety of human diseases are associated with abnormal lipid metabolism, such as obesity, diabetes mellitus, atherosclerosis, steatosis, Alzheimers disease, and cancer. The conservation of human orthologues in zebrafish makes this animal model a powerful genetic system to study vertebrate physiology. We utilize forward and reverse genetic approaches, combined with fluorescent lipid reporters, to search for genes that are involved in lipid metabolism in live zebrafish larvae. We have identified several mutants with altered lipid metabolism through this mutagenized screen. We have positionally cloned one of the mutants, fat-free (ffr), whose digestive system appeared morphologically normal despite exhibiting significantly diminished fluorescence in the digestive system (Farber et al. 2001, Science 262:1385 ). The ffr gene encodes a well-conserved protein with no previously known function while the fish ffr protein is approximately 75% identical to the human orthologue. Further phenotypic analyses suggest that ffr protein is involved in intracellular vesicular trafficking (Ho et al. 2006, Cell Metab.3:289-300). Abnormal vesicular trafficking is the origin or manifestation of many human diseases, including obesity, atherosclerosis, steatosis, diabetes mellitus, Alzheimers disease, and cancer. We currently use molecular, biochemical, genetic, and physiological approaches to elucidate the mechanisms that regulate lipid metabolism via manipulating ffr levels and functions. Our long-term research objective is to provide novel therapeutic strategies for preventing or treating human disease with abnormal lipid metabolism.
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Keywords
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Zebrafish; Forward genetics; Reverse genetics; Lipid Metabolism; Nutrition; Positional cloning; Intracellular vesicular trafficking; Developmental Biology
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Publications
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- Zoeller, J.J., Pimtong, W., Corby, H., Goldoni, S., Iozzo, A. E., Owens, R.T., Ho, S.Y., and Iozzo, R.V. (2009). A central role for decorin during vertebrate convergent extension. J.Biol. Chem. 284:11728-11737.
- Zoeller, J.J., McQuillan A., Whitelock, J., Ho, S.Y., and Iozzo, R.V. (2008) A central function for perlecan in skeletal muscle and cardiovascular development. J. Cell Biol. 181:381-394.
- Ouyang,M., Garnett, A.T., Han, T.M., Hama, K., Lee, A., Deng, Y., Lee, N., Liu, H.-Y., Amacher, S.L., Farber, S.A., and Ho, S.Y. (2008) A web based resource characterizing the zebrafish developmental profile of over 16,000 transcripts. Gene Expr. Patterns 8:171-180.
- Ho,S.Y., Lorent K., Pack M., and Farber S.A.(2006) Zebrafish fat-free is required for intestinal lipid absorption and Golgi apparatus structure. Cell Metab. 3:289-300.
- Ho,S.Y., Thrope, J, Deng, Y., Sonanta, E., DeRose, R. and Farber,S.A. (2004) Lipid metabolism in zebrafish. Methods in cell biology. 76:87-108
- Thorpe, J.L., Doitsidou, M., Ho, S.Y., Ras, E., and Farber, S.A. (2004) Germ cell migration in zebrafish is dependent on HMG-CoA reductase activity and prenylation. Dev. Cell 6:295-302.
- Rubinstein A.L., Ho S.Y., Farber, S.A. (2004). Zebrafish Model of Lipid Metabolism for Drug Discovery. In: Unravelling Lipid Metabolism with Microarrays. Berger A and Roberts M, eds. (Marcel Dekker Incorporated, New York), Ch. 19, pp. 423-432
- Ho, S.Y., Farber, S.A., and Pack, M. (2003) "Lipid metabolism and signaling in zebrafish " in "Model Organism in Drug Discovery".Carroll P.M. and Fitzgerald K edit. John Wiley & Sons Ltd, Weat Sussex, England. p203-221
- Ho,S.Y., Pack, M., and Farber, S.A. (2003) Analysis of small molecule metabolism in zebrafish. Methods in enzymology. 364:408-426
- Ho, S.Y., Delgado, L., and Storch, J. (2002) Monoacylglycerol metabolism in human intestinal Caco-2 cells: evidence for compartmentation and hydrolysis. J. Biol. Chem. 277:1816-1823
- Farber, S.A., Pack, M., Ho, S.Y., Johnson, E.K., Wagner, D.S., Dosch, R., Mullins, M.C.,Hendrickson, H.S., Hendrickson, I.D., Halpern, M.E. (2001) Genetic analysis of digestive physiology using fluorescent phospholipid reporters. Science 292:1385-1388
- Ho, S.Y., and Storch, J. (2001) Common mechanisms of monoacylglycerol and fatty acid uptake by human intestinal Caco-2 cells. Am. J. Physiol. 281:C1106-C1117
- Trotter, P.J., Ho, S.Y., and Storch, J. (1996) Fatty acid uptake by Caco-2 human intestinal cells. J. Lipid Res. 37:336-346
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Individual Expertise profile of
Shiu-Ying Ho, Ph.D., Copyright © Shiu-Ying Ho, Ph.D..
Last Updated
by Shiu-Ying Ho, Ph.D. : Saturday, April 18, 2009 2:09:11 PM
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