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Thomas Jefferson University - Carlisle Landel, Ph.D.
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Carlisle Landel, Ph.D.
Microbiology and Immunology
Thomas Jefferson University
Jefferson Medical College
Department of Microbiology and Immunology
Research Assistant Professor
Appointed 2005
Director
Transgenic and Gene Targeting Facility
Kimmel Cancer Center
Appointed 2005
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Mailing Address
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233 South 10th St, BLSB 619
Philadelphia, Pennsylvania 19107
United States
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Qualifications
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Ph.D., University of Colorado Boulder, Molecular, Cellular and Developmental Biology, 1982
A.B., University of California Santa Cruz, Biology, 1975
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Expertise and Research Interests
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Expertise: Design, production, maintenance, analysis and cryopreservation of transgenic mice; mouse genetics.
Research Interests: Cryopreservation of mouse sperm is a potentially valuable method for archiving valuable strains of mice. It not only provides insurance against loss of the strain, but also frees resources being, since a strain could be maintained from the freezer and retrieved only when needed rather than as a live colony that must be continually bred. The utility of this approach, however, is burdened by the fact that sperm from many inbred strains, and C57BL/6 in particular, do not survive cryopreservation. The ability of sperm from inbred strains of mice to survive cryopreservation is regulated by an as-yet unidentified series of genes. The focus of my research is to identify these genes as a prelude to understanding the biological causes of this phenomenon and also identify potential solutions. It also has broader implications in the medical and agricultural fields, since individual sperm donors, whether human or animal, will often produce semen samples that do not survive cryopreservation.
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Keywords
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mouse genetics; transgenic mice; mouse; mice; genetics; cryopreservation; reproductive biology; sperm; embryo; embryonic stem cells; transgenic rats
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Publications
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- Selected peer-reviewed publications (in chronological order).
- Landel CP, Krause M, Waterston RH, and Hirsh D. 1984. DNA rearrangements of the actin gene cluster in Caenorhabditis elegans accompany reversion of three muscle mutants. J Mol Biol 180(3):497-513.
- Lakshmanan J and Landel CP. 1986. Neonatal hyperthyroidism impairs epinephrine-provoked secretion of nerve growth factor and epidermal growth factor in mouse saliva. Pediatr Res 20(7):587-592.
- Chen S, Botteri F, van der Putten H, Landel CP, and Evans GA. 1987. A lymphoproliferative abnormality associated with inappropriate expression of the Thy-1 antigen in transgenic mice. Cell 51(1):7-19.
- Landel CP, Zhao J, Bok D, and Evans GA. 1988. Lens-specific expression of recombinant ricin induces developmental defects in the eyes of transgenic mice. Genes Dev 2(9):1168-1178.
- Landel CP, Chen SZ, and Evans GA. 1990. Reverse genetics using transgenic mice. Annu Rev Physiol 52:841-851.
- Ingold AL, Landel CP, Knall C, Evans GA, and Potter TA. 1991. Co-engagement of CD8 with the T cell receptor is required for negative selection. Nature 352(6337):721-723.
- Landel CP. 1991. The production of transgenic mice by embryo microinjection. Genet Anal Tech Appl 8(3):83-94.
- Brent RL, Beckman DA, and Landel CP. 1993. Clinical teratology. Curr Opin Pediatr 5(2):201-211.
- Sipes SL, Medaglia MV, Stabley DL, DeBruyn CS, Alden MS, Catenacci V and Landel CP. 1996. A new MHC Class Ib gene expressed in the mouse blastocyst and placenta. Immunogenetics 45(2):108-120.
- Landel CP, Stabley DL, and Bundesen LQ. 1997. PCR identification of class I major histocompatibility complex genes transcribed in mouse blastocyst and placenta. J Reprod Immunol 33(1):31-43.
- Landel, C.P. (2005) Archiving mouse strains by cryopreservation. Lab Animal, 34: 50-57.
- Borghei A. Ouyang YB. Westmuckett AD. Marcello MR. Landel CP. Evans JP. Moore KL. 2006 Targeted disruption of tyrosylprotein sulfotransferase-2, an enzyme that catalyzes post-translational protein tyrosine O-sulfation, causes male infertility. J.Biol Chem, 281(14):9423-31, 2006 Apr 7.
- Zhou H. Huang C. Yang M. Landel CP. Xia PY. Liu YJ. Xia XG. 2009. Developing tTA transgenic rats for inducible and reversible gene expression. Int J Biol Sci [Electronic]. 5(2):171-81, 2009.
- Book Chapters
- Smith RA, Landel CP, Cornelius CE and Revel M. 1986. Mapping the action of interferon on primate brain. In: The Biology of the Interferon System, Stewart II WE and Schellenkens H, eds. Elsevier, Amsterdam 563-566.
- Landel CP, Chen S, Botteri F, van der Putten H and Evans GA. 1989. Inappropriate expression of Thy-1 in transgenic mice causes a lympho-proliferative abnormality. In: Gene Transfer and Gene Therapy, UCLA Symposia on Molecular and Cellular Biology, New Series. Verma I, Mulligan R, and Beaudet A (eds). Alan R Liss, Inc., New York 87:179-188.
- Boggess, D, Silva, KA, Landel, CP, Mobraaten, L, and Sundberg, JP. 2004. Approaches to handling, breeding, strain preservation, genotyping and using drug administration for mouse models of cancer. In: Mouse Models of Human Cancer, Holland, EC (ed), John Wiley & Sons, Inc., New York. 3-14.
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Individual Expertise profile of
Carlisle Landel, Ph.D., Copyright © Carlisle Landel, Ph.D..
Last Updated
by Carlisle Landel, Ph.D. : Friday, September 18, 2009 11:07:29 AM
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