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Thomas Jefferson University - Hui Zhang, M.D., Ph.D.
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Hui Zhang, M.D., Ph.D.
Medicine
Thomas Jefferson University
Jefferson Medical College
Department of Medicine
Division of Infectious Diseases
Center for Human Virology
College of Graduate Studies
Ph.D Program in
Immunology and Microbial Pathogenesis
Professor
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Mailing Address
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1015 Walnut Street, Curtis Building 809
Philadelphia, Pennsylvania 19107
United States
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Qualifications
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M.D. 1982. Zhongshan School of Medicine, Sun Yatsen University, China
Ph.D. 1994. Medical Center at Syracuse, State University of New York, , Microbiology and Immunology.
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Expertise and Research Interests
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Dr. Hui Zhang's laboratory studies HIV-1 pathogenesis and therapeutics. In particular, this lab has focused on the function of an anti-HIV-1 cellular factor, APOBEC3G. They have shown that APOBEC3G, a cytidine deaminase, edits HIV-1 nascent reverse transcripts and induces G to A hypermutation. They have also shown that APOBEC3G is up-regulated by interferon-alpha through classical pathways in various primary cells. Therefore, APOBEC3G belongs to the IFN antiviral system. In addition, they have found that P-body-associated APOBEC3G inhibits the function of various cellular microRNAs.
Recently, this lab has found that a cluster of cellular miRNAs contributes to HIV-1 latency by interacting with the 3'-end of HIV-1 RNA. This interaction leads to the translational inhibition of various HIV-1 proteins, including Tat and Rev. The shortage of Tat and Rev further enforces HIV-1 latency. Therefore, reversal of HIV-1 latency could be achieved by manipulating cellular microRNAs.
The long term goals of this laboratory are to
(1) Study the effect of microRNAs on HIV-1 replication and search for the microRNA-related activators for HIV-1 latency;
(2). Further investigate the molecular mechanism how APOBEC3G inhibits HIV-1 replication
(3). Define the self-renewal capacity of T-cells during HIV-1 replication.
Currently, this lab is supported by a NIH RO1 grant (2009-2013) and a grant from a private biotech company
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Keywords
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HIV-1 Pathogenesis; APOBEC3G; Vif; Interferon; Innate Immunity; MicroRNAs; Viral Reservoirs; High-throughput Screening Assay for Antiviral Agents; Microbicides; Reverse Transcription; Proteomics; Nanoparticles
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Publications
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- Lin HT, Zhang ZP, Zhang H, Yang PS, Wang QL, and Bai L: Primary culture of human blood-retina barrier cells and preliminary study of APOBEC3 expression: an in vitro study. Investigative ophthalmology & visual science. 2009 Apr 15. [Epub ahead of print]
- Zhang H. Role of microRNA in HIV-1 latency (Invited review). Future HIV Therapy. 2008. 2: 281-290.
- Bai L, Zhang ZP, Zhang H, Yu, Q, Lin HT, and Yang W. HIV-1 Tat protein alters the tight junction protein expression and barrier function of retinal pigment epithelium. BMC Infectious Diseases. 2008. 8:77.
- Zhang H. Reversal of HIV-1 latency by anti-microRNA inhibitors. (Invited review). International Journal of Biochemistry and Cell Biology. 2008 Aug 8. [Epub ahead of print].
- Wang YJ, Wang X, Zhang H, Zhou L, Liu S, Kolson DL, Song L, Ye L, Zhang T, and Ho WZ. Expression and regulation of antiviral protein APOBEC3G in human neuronal cells. Journal of Neuroimmunology. 2008 Nov 20. [Epub ahead of print].
- Wang FX, Huang J, Zhang HG, Ma X, Zhang H. (2008) APOBEC3G upregulation by alpha interferon restricts human immunodeficiency virus type 1 infection in human peripheral plasmacytoid dendritic cells., Journal of General Virology, 89, 722-30
- Smith JA, Wang FX, Zhang H, Wu KJ, Williams KJ, Daniel R. (2008) Evidence that the Nijmegen breakage syndrome protein, an early sensor of double-strand DNA breaks (DSB), is involved in HIV-1 post-integration repair by recruiting the ataxia telangiectasia-mutated kinase in a process similar to, but distinct from, cellul, Virology Journal, 5, 11
- Huang J, Liang Z, Yang B, Tian H, Ma J, Zhang H. (2007) Derepression of microRNA-mediated protein translation inhibition by apolipoprotein B mRNA-editing enzyme catalytic polypeptide-like 3G (APOBEC3G) and its family members. Journal of Biological Chemistry, 282 (46), 33632-40
- Argyris EG, Acheampong E, Wang F, Huang J, Chen K, Mukhtar M, Zhang H. (2007) The interferon-induced expression of APOBEC3G in human blood-brain barrier exerts a potent intrinsic immunity to block HIV-1 entry to central nervous system., Virology, 367 (2), 440-51
- Huang J, Wang F, Argyris E, Chen K, Liang Z, Tian H, Huang W, Squires K, Verlinghieri G, Zhang H (2007) Cellular microRNAs contribute to HIV-1 latency in resting primary CD4+ T lymphocytes. Nature Medicine, 13 (10), 1241-7
- Yang B, Chen K, Zhang C, Huang S, Zhang H. (2007) Virion-associated uracil DNA glycosylase-2 and apurinic/apyrimidinic endonuclease are involved in the degradation of APOBEC3G-edited nascent HIV-1 DNA. Journal of Biological Chemistry, 282 (16), 11667-75
- Argyris EG, Dornadula G, Nunnari G, Acheampong E, Zhang C, Mehlman K, Pomerantz RJ, Zhang H. (2006) Inhibition of endogenous reverse transcription of human and nonhuman primate lentiviruses: potential for development of lentivirucides. Virology, 353 (2), 482-90
- Chen K, Huang J, Zhang C, Huang S, Nunnari G, Wang FX, Tong X, Gao L, Nikisher K, Zhang H. (2006) Alpha interferon potently enhances the anti-human immunodeficiency virus type 1 activity of APOBEC3G in resting primary CD4 T cells. Journal of Virology, 80 (15), 7645-57
- Nunnari G, Xu Y, Acheampong EA, Fang J, Daniel R, Zhang C, Zhang H, Mukhtar M, Pomerantz RJ. (2005) Exogenous IL-7 induces Fas-mediated human neuronal apoptosis: potential effects during human immunodeficiency virus type 1 infection. Journal of Neurovirology, 11 (4), 319-28
- Fang J, Kubota S, Yang B, Zhou N, Zhang H, Godbout R, Pomerantz RJ. (2004) A DEAD box protein facilitates HIV-1 replication as a cellular co-factor of Rev., Virology, 330 (2), 471-80
- Gallego J, Greatorex J, Zhang H, Yang B, Arunachalam S, Fang J, Seamons J, Lea S, Pomerantz R, Lever AM. Rev binds specifically to a purine loop in the SL1 region of the HIV-1 leader RNA. Journal of Biological Chemistry. 278: 40385-91.(2003)
- Zhang H, Yang B, Pomerantz RJ, Zhang C, Arunachalam SC, Gao L. The cytidine deaminase CEM15 induces hypermutation in newly synthesized HIV-1 DNA. Nature. 424(6944): 94-8, (2003)
- Yang B, Gao L, Li L, Lu Z, Fan X, Patel CA, Pomerantz RJ, DuBois GC, Zhang H. Potent suppression of viral infectivity by the peptides that inhibit multimerization of human immunodeficiency virus type 1 (HIV-1) Vif proteins. Journal of Biological Chemistry. 278(8): 6596-602, (2003)
- Di Mascio M, Dornadula G, Zhang H, Sullivan J, Xu Y, Kulkosky J, Pomerantz RJ, Perelson AS. In a subset of subjects on highly active antiretroviral therapy, human immunodeficiency virus type 1 RNA in plasma decays from 50 to <5 copies per milliliter, with a half-life of 6 months. Journal of Virology. 77(3): 2271-5, (2003)
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Individual Expertise profile of
Hui Zhang, M.D., Ph.D., Copyright © Hui Zhang, M.D., Ph.D..
Last Updated
by Hui Zhang, M.D., Ph.D. : Tuesday, July 28, 2009 12:08:35 PM
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