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Thomas Jefferson University - Gino Cingolani, Ph.D.
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Qualifications
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B.S./M.S. in Biochemistry, University of Bari, Italy, 1995
Ph.D. in Structural Biology, European Molecular Biology Laboratory, 1999
Human Frontier Science Program Postdoctoral fellow, Scripps Research Institute, 1999-2003
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Expertise and Research Interests
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My laboratory uses X-ray crystallography, combined with biophysical and biochemical techniques to probe the structure and function of large macromolecular machines. We are interested in a variety of biological problems, which include the mechanisms and regulation of nucleocytoplasmic transport, the delivery of viral DNA into living cells, and the structure and regulation of multi-subunit ATP synthases. Our structural approach provides a unique tool to understand the function and engineer the activity of biological molecules. Visit our website (www.cingolanilab.org) for more info.
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Keywords
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X-ray crystallography; Structural Biology; Nucleocytoplasmic Transport; Dual Specificity Phosphatase; Virus Assembly; DNA Injection; ATP synthase; Macromolecular Machines
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Publications
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- Bhardwaj, A., Walker-Kopp, N., Casjens, S.R., and Cingolani, G. (2009) An evolutionarily conserved family of virion tail needles related to bacteriophage P22 gp26: correlation between structural stability and length of the a-helical trimeric coiled-coil. J. Mol. Biol. 391(1):227-45.
- Mitrea, D.M., Butler, J.S., Mitrousis, G., Cingolani, G. and Loh, S. N. (2009). Structural and Thermodynamic Analysis of Conformationally-Strained Circular Permutants of Barnase. Biochemistry. 48(15):3497-507.
- Koksal, A., Nardozzi, J., and Cingolani, G. (2009) Dimeric quaternary structure of the prototypical dual specificity phosphatase VH1. J. Biol. Chem. 284(15):10129-37.
- Olia, S.A., Casjens, S., and Cingolani, G. (2009) Structural Plasticity of the phage P22 tail-needle gp26 probed with Xenon Gas. Protein Science. 18(3):537-548.
- Olia, S.A., and Cingolani, G. (2008) A shifty stop for a hairy tail. Mol. Microbiology, 70(3):549-53.
- Bhardwaj, A., Walker-Kopp N, Wilkens, S., and Cingolani, G. (2008) Foldon-guided self-assembly of ultra stable protein fibers. Protein Science 17(9):1475-85.
- Lorenzen, K., Olia, S.A., Uetrecht, C., Cingolani, G., and Heck, A.J.R. (2008). Determination of stoichiometry and conformational changes in the first step of the P22 tail assembly. J. Mol. Biol. 379(2):385-96.
- Mitrousis, G., Olia, S.A., Walker and Cingolani, G. (2008). Molecular basis for the recognition of snurportin 1 by importin b. J. Biol. Chem. 283(12):7877-84.
- Zheng, H., Olia, S.A., Hankins, M., Andrews, S., Cingolani, G., and Gonen, T, (2008). A conformational switch in bacteriophage P22 portal protein primes genome injection. Molecular Cell. 29(3), 376-383.
- Olia, S.A., Casjens, S. and Cingolani, G. (2007) Structure of Phage P22 Cell Envelope Penetrating Needle. Nature Struc Mol Biol. 14(12), 1221-1226.
- Olia, S.A., Bhardwaj, A., Joss, L., Casjens, S., and Cingolani, G. (2007) The role of gene 10 protein in the hierarchical assembly of the bacteriophage P22 portal vertex structure. Biochemistry. 46(30), 8776-8784.
- Bhardwaj, A., Olia, S.A., Walker-Kopp N, and Cingolani, G. (2007) Domain organization and polarity of tail needle gp26 in the portal vertex structure of bacteriophage P22. J. Mol. Biol. 371(2), 374-87.
- 17. Olia, A., Al-Bassam, J., Winn-Stapley, D.A., Joss, L., Casjens, S., and Cingolani, G. (2006) Binding induced stabilization and oligomerization of portal closure factor gp4 in the assembly pathway of phage P22 tail. J. Mol. Biol. 363, 558576.
- Cingolani, G., Andrews, D., and Casjens, S. (2006) Crystallogenesis of bacteriophage P22 tail accessory factor gp26 at acidic and neutral pH. Acta Cryst. F 62, 477482.
- Andrews D, Butler JS, Al-Bassam J, Joss L, Winn-Stapley DA, Casjens S, and Cingolani, G. (2005) Bacteriophage P22 Tail Accessory Factor GP26 Is a Long Triple-stranded Coiled-coil. J. Biol. Chem. 280(7): 5929-33.
- Chen MH, Ben-Efraim I, Mitrousis G, Walker-Kopp N, Sims PJ, and Cingolani, G. (2005) Phospholipid scramblase 1 (PLSCR1) contains a non-classical nuclear localization signal with unique binding site in importin a. J. Biol. Chem. 280(11):10599-606.
- Tang L., Marion W., Cingolani, G., Prevelige P.E. Jr., and Johnson J.E. (2005) The three-dimensional structure of the tail machine of bacteriophage P22. EMBO J. 24(12):2087-95.
- Wodrich, H., Guan, T., Cingolani, G., von Seggern, D., Nemerow, G., and Gerace, L. (2003). Switch from capsid protein import to adenovirus assembly by cleavage of nuclear transport signals. EMBO J. 22 (23): 6150-6162.
- Bednenko, J., Cingolani, G., and Gerace, L. (2003). Importin b contains a COOH-terminal nucleoporin binding region important for nuclear import. J. Cell Biol. 162(3): 391-401.
- Koerner, C., Guan, T., Gerace, L. and Cingolani, G. (2003). Synergistic effects of silent and hot spot mutations in importin b reveals a dynamic mechanism for recognition of a nuclear localization signal. J. Biol. Chem. 278 (18): 16216-16221.
- Cingolani, G., Bednenko, J., Gillespie, M., and Gerace, L. (2002). Molecular basis for the recognition of a non-classical nuclear localization signal (NLS) by importin b. Molecular Cell, 10: 1345-1353.
- Cingolani, G., Lashuel, H., Gerace, L., and Mueller, C.W. (2000). Nuclear import factors importin a and importin b undergo mutually induced conformational changes upon association. FEBS Lett., 484(3): 291-298.
- Cingolani, G., Petosa, C., Weis, K., and Mueller, C.W. (1999). Structure of importin b bound to the IBB domain of importin a. Nature, 399(6733): 221-229.
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Individual Expertise profile of
Gino Cingolani, Ph.D., Copyright © Gino Cingolani, Ph.D..
Last Updated
by Admin
: Tuesday, November 3, 2009 11:01:35 AM
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