James H. Keen, PhD
Philadelphia, PA 19107
(215) 503-0622 fax
Most Recent Peer-reviewed Publications
- Arf6 regulation of Gyrating-Clathrin
- GGA3 functions as a switch to promote met receptor recycling, essential for sustained ERK and cell migration
- Development and application of in vivo molecular traps reveals that dynein light chain occupancy differentially affects dynein-mediated processes
- Gyrating clathrin: Highly dynamic clathrin structures involved in rapid receptor recycling
- Interaction of prostate specific membrane antigen with clathrin and the adaptor protein complex-2
PhD, Cornell University, Biochemistry - 1976
AB, Cornell University, Government (Chemistry) - 1969
Postdoctoral Fellow, NIH-NCI (Bethesda, MD), 1977-1979
Rothschild-Yvette Mayent Fellow, Institut Curie, Paris, France, 2010
Dean, Jefferson College of Graduate Studies, Vice-President for International Affairs, Thomas Jefferson University, Philadelphia, PA, 2003-2010
Director, Kimmel Cancer Center Bioimaging Facility, Thomas Jefferson University, Philadelphia, PA 1991-present
Member, ASBMB Mentoring Committee, 2011 _ present.
Associate Editor: Public Library of Science One (PLoS ONE), 2007- present
Associate Editor, Receptor, 1991- 2003
Keynote Lecture, Membrane Research Forum of the Membrane Organizer Project, Advanced Technology Organization, JST, Nagoya, Japan, 2002
Organizer, 10th Annual Growth Factor and Signal Transduction Conference on Endocytosis and Intracellular Trafficking, Iowa State Univ., 1998
NIH Board of Scientific Counselors, National Heart, Lung and Blood Institute, 1990, 1994, 1997.
NIH Board of Scientific Counselors, Promotion Review Board, Division of Computer Research and Technology, NIH, 1994-1995
Expertise & Research Interests
The long-term goal of my lab has been an understanding of the structure of clathrin-coated membranes and their role in membrane transport processes within mammalian cells. It is well established that plasma membrane coated pits mediate endocytosis, and that clathrin coated buds and tubules in the TGN are involved in targeting and recycling of newly synthesized proteins. But clathrin coats are also present on vacuolar and tubular domains of endosomes where they are involved in sorting to late endosomes, or to retrograde or recycling pathways, respectively, through mechanisms that are only partially understood. As the early/sorting endosome is a nexus for virtually all extracellular materials entering cells as well as many intracellular trafficking cargoes, understanding sorting mechanisms and structures in the endosomal region remains a key challenge with many implications for human health and disease. In this context, we have identified and characterized distinct clathrin coated structures in the endosomal region that exhibit extremely rapid but localized movement. Accordingly we termed these structures 'Gyrating'- or 'G-clathrin', and they appear to be clathrin coated buds along or on the ends of membrane tubules. We have shown that G-clathrin accumulates internalized cargo destined to be recycled to the plasma membrane, including transferrin and c-Met receptor, and that they function in rapid recycling from the endosome which remains a largely uncharacterized pathway. In recent work, we have shown that the dynamics of G-clathrin requires completion of the Arf6 guanine nucleotide cycle but that Arf1 can also support its formation. Similarly, both BFA-sensitive and -resistant guanine nucleotide exchange (GEF) proteins modulate G-clathrin levels, together indicating that overlapping mechanisms are operative in G-clathrin regulation. The complexity of regulation appears to parallel the multiplicity of endosomal sorting pathways.
Current efforts utilize two major approaches. First, we are dissecting the regulation and function of G-clathrin in rapid recycling pathways and identifying the cargos transported, with a focus on the role of GGA proteins and other potential regulators and adaptors. Second, we are interested in better visualizing the structure and function of G-clathrin in intact cells, using correlative light-electron microscopy (CLEM) in collaborative efforts with the lab of Graça Raposo (Institut Curie, Paris) at the ultrastructural level, and interactions and interconversions of G-clathrin with other endocytic and membrane compartments using high-speed live cell imaging.
Our methodological approaches utilize widefield, TIRF and spinning disk confocal fluorescence microscopy and associated photobleaching/photoactivation and image analysis procedures, the development and application of novel "molecular traps" to probe functions in intact cells in collaboration with John Williams (City of Hope, Duarte CA), and conventional cell and molecular biology techniques such as siRNA-mediated protein depletion and exogenous expression, and others.
Biochemistry; Biophysics; Cell Biology; Developmental Biology; Genetics; Human Physiology; Pharmacology; neuron; antibody; clathrin; mast cell; exocytosis; macrophage; radiotracer; calcium flux; cytoskeleton; cell membrane; stoichiometry; immune complex; molecular site; phosphorylation; laboratory mouse; light microscopy; membrane protein; western blotting; laboratory rabbit; membrane activity; membrane structure; electron microscopy; membrane biogenesis; tissue cell culture; phosphatidylinositol; protein biosynthesis; nucleic acid sequence; fluorescence microscopy; site directed mutagenesis; platelet activating factor; protein structure function; confocal scanning microscopy; receptor mediated endocytosis