Dr. Anni investigates the reprogramming of key regulatory transcriptional networks by ethanol at the onset of embryonic development in Fetal Alcohol Spectrum Disorder (FASD) using a mouse embryonic stem cell model and an integrated experimental and computational approach encompassing single-cell multiplex flow cytometry, RT-qPCR, microfluidic chips, immunocytochemistry, immunoblotting, mechanistic perturbations and bioinformatic analysis. I have a broad background in ethanol-mediated injury, with experience in key research areas to successfully carry out the proposed work. As a postdoctoral fellow at the University of Pennsylvania, I was trained in several biophysical and biochemical techniques (fluorescence and Raman spectroscopy, ESR, NMR, EXAFS, stopped flow). At Thomas Jefferson University, I expanded my skills to mass spectroscopy to identify proteomic biomarkers of alcoholism. As a PI or co-Investigator on several NIH-funded grants, I developed workflows for screening phage-displayed peptides, synthetic antisense oligonucleotides and small molecules as specific enzyme inhibitors in vitro and in vivo. I successfully administered all aspects of projects, including staffing, budget, progress reports, research protections, and produced peer-reviewed publications. I have a record of applying innovative experimental approaches in projects focusing in ethanol-mediated injury. My laboratory has been offering training in these areas to undergraduate, graduate students and postdoctoral fellows.
- Ethanol alters the balance of Sox2, Oct4, and nanog expression in distinct subpopulations during differentiation of embryonic stem cells
- An Optimized Method for the Measurement of Acetaldehyde by High-Performance Liquid Chromatography
- Ethanol effects on transcription factor network regulating stem cell differentiation
- Suppression of heavy drinking and alcohol seeking by a selective ALDH-2 inhibitor
- Oligonucleotide-mediated gene targeting in human hepatocytes: Implications of mismatch repair