Joseph Rabinowitz, Ph.D.

Contact Dr. Rabinowitz

Center for Translational Medicine
1025 Walnut Street, Suite 411
Philadelphia, PA 19107

(215) 503-5590
(215) 503-5731 fax

Degree
Ph.D., Case Western Reserve University, Cleveland, Ohio 1995

University Appointment
Assistant Professor, 2004

Research and Clinical Interests
Dr. Rabinowitz's laboratory is most interested in increasing the transduction efficiency of viral gene delivery vehicles derived from the human parvovirus Adeno-associated virus (AAV). This virus is a small (20-30nm) non-enveloped non-pathogenic single stranded DNA containing dependovirus in the parvovirus family. Multiple AAV serotypes have been isolated over the last forty years and each of these serotypes has a tendency to transduce specific tissue targets. Additionally, each of these serotypes transduces tissues with a different efficiency. One goal of this laboratory is to determine domains on the viral shells of the different AAV serotypes, which govern their distinct tropisms. This lab is also interested in altering those domains so the normal viral tropisms are reduced so that when tropism determining epitopes are added to the virus shell a new target is acquired.

Publications

Most Recent Peer-reviewed Publications

1. Analysis of AAV serotypes 1-9 mediated gene expression and tropism in mice after systemic injection
2. Stable myocardial-specific AAV6-S100A1 gene therapy results in chronic functional heart failure rescue
3. Adeno-associated virus serotypes 1 to 5 mediated tumor cell directed gene transfer and improvement of transduction efficiency
4. Remodeling of cortical bone allografts mediated by adherent rAAV-RANKL and VEGF gene therapy
5. Differential myocardial gene delivery by recombinant serotype-specific adeno-associated viral vectors
6. Autoimmune anemia in macaques following erythropoietin gene therapy
7. Cross-Dressing the Virion: The Transcapsidation of Adeno- Associated Virus Serotypes Functionally Defines Subgroups
8. Optimal design of a single recombinant adeno-associated virus derived from serotypes 1 and 2 to achieve more tightly regulated transgene expression from nonhuman primate muscle
9. Recombinant adeno-associated virus serotype 4 mediates unique and exclusive long-term transduction of retinal pigmented epithelium in rat, dog, and nonhuman primate after subretinal delivery
10. Marker rescue of adeno-associated virus (AAV) capsid mutants: A novel approach for chimeric AAV production
11. Crystal-clear view
12. The adeno-associated virus crystal: Impact inversely proportional to size
13. Looking into the eclipse
14. Cross-packaging of a single adeno-associated virus (AAV) type 2 vector genome into multiple AAV serotypes enables transduction with broad specificity
15. Building a better vector: The manipulation of AAV virions
16. Several log increase in therapeutic transgene delivery by distinct adeno-associated viral serotype vectors
17. Insertional mutagenesis of AAV2 capsid and the production of recombinant virus
18. Adeno-associated virus expression systems for gene transfer
19. Targeted mutation of Ncam to produce a secreted molecule results in a dominant embryonic lethality
20. Independent gene targeting by coelectroporation of multiple vectors

View All Publications