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Services offered by the Genomics Core
Services
offered by the Genomics Core
Our list of available services evolves with the changing needs
of investigators.
 Affymetrix GeneChip System
DNA microarray technology is one of the most promising analytical
techniques to identify single-nucleotide polymorphisms and mutations,
small insertions and deletions and to assess gene copy number and
genome-wide mRNA expression patterns.
The Affymetrix GeneChip System consists of a hybridization oven,
fluidics station, the Gene Chip Scanner 3000, and a computer workstation
with GCOS v1.1 software for controlling the system and basic data
analysis functions.
mRNA expression profiling for definition of expressed and differentially
expressed genes are available.
SNP profiling and determination of loss of heterozygosity is available
via the 10K, 100K and GenFlex arrays.
Third-party software packages (GeneSpring, Spotfire, Probe Profiler,
etc.) are available for performing gene clustering and construction
of self-organizing maps.
A Core Lab package permits the DNA Sequencing/Genetic Analysis
Facility to distribute copies of the Microarray Analysis Suite
software to investigators who want to perform basic analysis of
Affymetrix chip data on their own computers. A workstation includes
the basic chip analysis software, a data mining tool, and the MicroDB
data management system.
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Affymetrix GeneChip Arrays
All GeneChip arrays available from Affymetrix can be run in the
Center. Commonly run expression arrays are: Human, Mouse, Rat,
mtDNA, Zebrafish, C. elegans, Yeast, and Drosophila. The 10K and
100K arrays are used for mapping and linkage analysis.
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 Agilent 2100 BioAnalyzer
The 2100 BioAnalyzer is a microfluidic-based platform using lab-on-a-chip
technology to provide quantitative and qualitative analysis of
DNA, RNA, proteins and cells. Up to 12 samples can be run on one
chip.
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ABI Prism 7900HT
The ABI Prism 7900HT Sequence Detection System is an automated,
high-throughput sequence detection system employing fluorescent PCR-related chemistries
such as real-time, end-point and dissociation curve analysis. The instrument is capable of running in one of three formats:
96-well, 384-well or in 384 -microfluidic card. The automation accessory allows for multiple
plate runs. Ready-to-use Assays-on-Demand are available for both SNP genotyping
and gene expression assays. ABI also provides an Assays-by-Design
service for new assay development.
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Pyrosequencing PSQ HS 96A
The Pyrosequencing system includes the instrument, system controller
and software for SNP and DNA mutation analysis.
This approach combines a DNA synthesis protocol for determining
DNA sequence using an enzyme cascade that generates light whenever
pyrophosphate is released during the template-mediated primer elongation
steps.
The PSQ HS 96A requires minimal amounts of sample (~1pmol).
A robotic arm is integrated into the system allowing for up to
10 plates to be run at one time.
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