Dessislava Markova, PhD
Curtis Building, Suite 501
Philadelphia, PA 19107
(215) 955-9773 fax
Most Recent Peer-reviewed Publications
- CCN2 suppresses catabolic effects of interleukin-1β through α5β1 and αVβ3 Integrins in nucleus pulposus cells: Implications in intervertebral disc degeneration
- Substance p stimulates production of inflammatory cytokines in human disc cells
- An organ culture system to model early degenerative changes of the intervertebral disc II: Profiling global gene expression changes
- COL6A3 protein deficiency in mice leads to muscle and tendon defects similar to human collagen VI congenital muscular dystrophy
- Expression and relationship of proinflammatory chemokine RANTES/CCL5 and cytokine IL-1 β in painful human intervertebral discs
PhD, Institute of Molecular Biology, Bulgarian Academy of Sciences, Sofia, Bulgaria - 1995
Expertise and Research Interests
Our laboratory is engaged in elucidating the molecular mechanisms of axial back pain.
Major research focus of our lab is to study the cytokine gene expression and protein levels in human discs. Our laboratory is currently studying the cytokine profile of the posterior annulus fibrosus (AF) from patients with degenerative disc disease( DDD). Chemical irritation or pressure on sinuvertebral nerve endings, which convey pain sensation from the posterior disc, has been implicated in the development of axial and lower extremity pain in patients with (DDD). Analysis of cytokine profiles of the posterior AF may help to elucidate inflammatory responses specific to this region of the disc in hopes of developing targeted therapies.
Another major research interest is to investigate and optimize the environmental conditions in an in vitro organ culture system that modulate the function of nucleus pulposus and annulus fibrosus cells to mimic early degenerative changes of the disc. Our laboratory is studying the molecular changes in expression of genes involved in matrix synthesis and turnover after the application of electrical stimulation to the in vitro rat organ cultured system. Results of this study will provide a detailed analysis of the effects of electrical stimulation on repair of the nucleus pulposus and annulus fibrosus cells at the cellular and molecular level. Moreover, the data will help to design a treatment strategy for future in vivo experiments, in terms of the dose, time and the optimum biological response.
Intervertebral disc, Annulus Fibrosus, Nucleus Pulposus, Disc degeneration, Chemokines, Cytokines , Growth Factors, Sensory neuropeptides.