Biddanda C. Ponnappa, PhD
Jefferson Alumni Hall, Suite 275
Philadelphia, PA 19107
Most Recent Peer-reviewed Publications
- Knockdown of Ki-67 by Dicer-Substrate Small Interfering RNA Sensitizes Bladder Cancer Cells to Curcumin-Induced Tumor Inhibition
- Dicer-substrate siRNA inhibits tumor necrosis factor alpha secretion in Kupffer cells in vitro: In vivo targeting of Kupffer cells by siRNA-liposomes
- siRNA for inflammatory diseases
- Inhibition of tumor necrosis factor alpha secretion in rat Kupffer cells by siRNA: In vivo efficacy of siRNA-liposomes
- Inhibition of tumor necrosis factor alpha secretion and prevention of liver injury in ethanol-fed rats by antisense oligonucleotides
PhD, University of Mysore - 1976
Research and Clinical Interests
Current research interests include a) development of liposome-mediated drug delivery system to target macrophages in vivo in animal model systems, b) construction of antisense oligonucleotides and siRNAs against inflammatory cytokines of macrophagic origin and c) to explore the therapeutic potential of curcumin, an herbal product, in the treatment of cancer and alcoholic liver disease.
Development and identification of delivery systems for the in vivo delivery of siRNAs and antisense oligonucleotides to macrophages. The goal of this project is to suppress the production of pro-inflammatory cytokines, such as interleukins and tumor necrosis factor alpha, which play a role in sepsis and many other inflammatory diseases including alcoholic liver disease. A liposome-based delivery system for the in vivo delivery of Dicer-substrate siRNA is in the final stages of completion.
To test the efficacy and the therapeutic potential of curcumin, a plant-based non-toxic polyphenol, against transitional cell carcinoma (TCC) of urinary bladder. An orthotopically implanted bladder cancer model has already been established in our laboratory for the rat model. Future studies also include development of water-soluble preparations of curcumin for in vivo delivery.
Familiar with various laboratory techniques including isolation hepatic cells, liposome preparation, fluorescence microscopy, gel electrophoresis, spectroscopy, Gas-Liquid-Chromatography, animal model systems and other routine laboratory techniques. Other areas include, grant writing and journal editing.
Since the liposomal formulations of antisense oligonucleotides and siRNAs of interest have therapeutic potential, our studies are of relevance to the pharmaceutical industry.