Thomas Jefferson UniversityJefferson Medical College

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Applied / Translational Genomics Shared Facility

The Applied / Translational Genomics Shared Facility in the Department of Pathology, Anatomy and Cell Biology provides equipments and technical support for automated nucleic acid purification (SPRI-TE) , DNA and RNA analysis (Agilent Bioanalyzer 2100), DNA fragmentation by sonication (Diagenode Bioruptor), automated MeDIP and ChIP (Diagenode IP-Star), real-time PCR (Roche Light-Cycler 480) for gene expression analysis, micro-RNA expression analysis, etc. It also offers microarray technology (both Agilent and Roche-Nimblegen arrays) and DNA next generation sequencing (Roche 454 platform for mutation/SNP detection and quantification, mRNA sequencing, etc). Bioinfomatic support is provided through the Daniel Baugh Institute of Computational Biology and Functional Genomics. The facility welcomes collaboration and can also assist users in related experimental design.

Instruments supporting the facility include the Roche 454 GS FLX and GS Junior Next Generation Sequencers, Agilent High-Resolution Microarray Scanner with SureScan technology, Diagenode IP-Star Automated System for ChIP and MeDIP, Diagenode Bioruptor sonicator, Roche LightCycler 480 RT PCR System, Beckman Coulter SPRI-TE Nucleic Acid Extractor, Agilent 2100 Bioanalyzer, Nimblegen Microarray Hybridization Station, and Agilent Microarray Hybridization Oven.

For further information contact:
Zoe Wang, PhD
(215) 955-0611

Jean-Marc Navenot, PhD
(215) 503-6250

Barry Evans
(215) 503-6114

Please drop all samples off at
1020 Locust Street, JAH Room 331

Applied / Translational Genomics Shared Facility Pricing
CGH 1x244k $1206
CGH 4x44k $1922
miRNA 8x15k $1533
Shotgun $2231
Amplicon $2000
Diagenode $3708
Shotgun mRNA seq $3440
CGH 4x44k (NR) $509
miRNA 8x15k $482
Methylation 3x720k (NR) $482
ChIP on Chip 3x720k (NR) $482
Shotgun (NR) $857
Amplicon (NR) $857
Diagenode (NR) $268
Shotgun mRNA seq (NR) $1232

Shared Molecular Facility Requisition Form (PDF)