Philadelphia University + Thomas Jefferson University

Transmission Electron Microscopy

The Research Electron Microscopy Laboratory in the Department of Pathology, Anatomy and Cell Biology aims to provide researchers with ultrastructure in specimen prepared from tissue, cellular or subcellular preparations. We are also open (on case by case base) to collaborative consultations to assist users in TEM related experimental design and analysis. Current capabilities/capacities allow 2D transmission electron microscopy.

The core instrument is an FEI Tecnai 12 120 keV digital TEM, equipped with an AMT XR111 CCD camera. The sample preparation laboratory has two Leica UCT ultramicrotomes.  

Services available include:

Providing training and supervision for the user to obtain images with the scope. Providing instructions/demo for routine and non-routine fixation and embedding. Limited scoping assignments pending on support personnel availability.

In the current setting specimen preparation (fixation, embedding) and obtaining (ultra)thin sections is the user’s responsibility (either by own or via other regional EM services).