J. Wesolowski, MM. Weber, A. Nawrotek, CA. Dooley, M. Calderon, CM. St. Croix, T. Hackstadt, J. Cherfils, F. Paumet (2017) “Chlamydia Hijacks ARF GTPases To Coordinate Microtubule Posttranslational Modifications and Golgi Complex Positioning” mBio 8: e02280-16
This study provides the first evidence that a chlamydial protein CT813 recruits the GTPases ARF1 and ARF4, which in turn play a critical role in controlling post-translationally modified microtubules around the inclusion. Furthermore, this study demonstrates that Chlamydia trachomatis hijacks this novel function of ARF to reposition the Golgi mini stacks during infection.
E. Ronzone, J Wesolowski, Laura D. Bauler, Anshul Bhardwaj, Ted Hackstadt, F. Paumet (2014) “An alpha-helical core encodes the dual functions of the chlamydial protein IncA” Journal of Biological Chemistry, 289
Here, the functional core of the SNARE-like protein IncA has been identified, which provides the basis for a more comprehensive understanding of its mechanisms of action and could aid in the development of novel therapeutics capable of interfering with its pathogenic function in vivo. This project provides the most detailed understanding of how a chlamydial inclusion protein operates to manipulate membrane fusion.
J. Wesolowski, F. Paumet (2014) “Escherichia coli exposure inhibits exocytic SNARE-mediated membrane fusion in mast cells” Traffic 15:516-530
This study demonstrates that E. coli exposure inhibits the formation of the exocytic SNARE complex and thus the release of granules. As a result, IgE-induced inflammatory reactions are significantly dampened. Ultimately, these results suggest that the microenvironment within which mast cells reside modulates their activation.
E. Ronzone, F. Paumet (2013) “Two coiled-coil domains of Chlamydia trachomatis IncA affect membrane fusion events during infection” PLoS One 8:e69769
These results demonstrate for the first time that Chlamydia employs SNARE-like virulence factors that positively and negatively affect membrane fusion and promote infection.