Thomas Jefferson University
Sidney Kimmel Medical College
Department of Medicine

Research Activity

Kiriakidou Lab

microRNAs in Systemic Lupus Erythematosus

RNA interference (RNAi) operates as a mechanism of innate immunity in plants, drosophila and C. elegans and depends on double stranded RNA and proteins of the Argonaute family. RNAi, programmed by exogenous small RNAs, is a powerful tool for gene-specific studies. The microRNA pathway intersects with the RNAi pathway and regulates gene expression in plants and animals. Animal microRNAs bind directly to Argonaute proteins (Ago1-4), providing target specificity to the regulatory function of Ago complexes. Research in the Kiriakidou lab focused on the biology of animal Ago proteins by studying the expression, regulation and function of animal Agos in human and mouse cells, mouse models and in vitro systems.

MicroRNAs are involved in numerous biological processes, and their expression is differentially regulated in human disease. Systemic Lupus Erythematosus (SLE, lupus) is a prototype systemic autoimmune disease characterized by autoantibody production and tissue deposition of immune complexes. Immunopathology in lupus is characterized by aberrant B and T cell functions. We are studying the role of microRNAs in pathways contributing to disease-specific B and T cell phenotypes and organ damage in SLE using microRNA KO models and mouse lupus models (congenic and induced). We also use synthetic inhibitors for microRNA silencing in vivo, to complement our genetic studies and to investigate potential use of microRNA.

Lab Members

Barry Garchow, PhD

Recent Publications

Colonoids from Patients with Pediatric Inflammatory Bowel Disease Exhibit Decreased Growth Associated with Inflammation Severity and Durable Upregulation of Antigen Presentation Genes

Tyrosine kinases in the pathogenesis of tissue fibrosis in systemic sclerosis and potential therapeutic role of their inhibition

Classification and prediction of post-trauma outcomes related to PTSD using circadian rhythm changes measured via wrist-worn research watch in a large longitudinal cohort

FcgRIIA expression accelerates nephritis and increases platelet activation in systemic lupus erythematosus

Chemical exposure-induced systemic fibrosing disorders: Novel insights into systemic sclerosis etiology and pathogenesis

One-Year Health Care Utilization and Recurrence After Incisional Hernia Repair in the United States: A Population-Based Study Using the Nationwide Readmission Database

Occupational Allergies to Cannabis

Health Assessment Questionnaire-Disability Index (HAQ-DI) use in modelling disease progression in diffuse cutaneous systemic sclerosis: an analysis from the EUSTAR database

Deep immune profiling of COVID-19 patients reveals distinct immunotypes with therapeutic implications

Impact of tumor necrosis factor inhibitors and methotrexate on diabetes mellitus among patients with inflammatory arthritis

Racial differences in systemic sclerosis disease presentation: A European Scleroderma Trials and Research group study

Functional deficits in mice expressing human interleukin 8

Musculoskeletal infection in orthopaedic trauma: Assessment of the 2018 international consensus meeting on musculoskeletal infection

NK cell–derived GM-CSF potentiates inflammatory arthritis and is negatively regulated by CIS

Homozygous IL1RN Mutation in Siblings with Deficiency of Interleukin-1 Receptor Antagonist (DIRA)

Self-reported baseline phenotypes from the International Fibrodysplasia Ossificans Progressiva (FOP) Association Global Registry

Long non-coding RNA HOTAIR drives EZH2-dependent myofibroblast activation in systemic sclerosis through miRNA 34a-dependent activation of NOTCH

Arp2/3 inactivation causes intervertebral disc and cartilage degeneration with dysregulated TonEBP-mediated osmoadaptation

Postoperative delirium after major orthopedic surgery

Recurrence of progressive skin involvement following discontinuation or dose reduction of Mycophenolate Mofetil treatment in patients with diffuse Systemic Sclerosis

A 72-Year-Old Man With a Violaceous Rash and Sepsis

Systematic review of recommendations on the use of methotrexate in rheumatoid arthritis

Socio-demographic and trauma-related predictors of PTSD within 8 weeks of a motor vehicle collision in the AURORA study

IgM Plasma Cells Reside in Healthy Skin and Accumulate with Chronic Inflammation

Role of microRNA in the pathogenesis of systemic sclerosis tissue fibrosis and vasculopathy