Thomas Jefferson University
Sidney Kimmel Medical College
Department of Medicine

Edelstein, Leonard

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Leonard Edelstein, PhD

Contact Dr. Edelstein

1020 Locust Street
Suite 394
Philadelphia, PA 19107

(215) 955-1797
(215) 955-9170 fax

Research Interests

Dr. Edelstein’s laboratory studies gene expression in megakaryocytes and platelets and their role in cardiovascular disease and thrombopoiesis. Current research includes:

  • Activated platelets release microparticles (MPs) which contain RNA and protein. These MPs have been shown to deliver miRNAs to endothelial cells in culture and alter the level of RNAs in these cells. We are studying the effect of MP-delivered miRNAs  on endothelial cell biology.

  • Genome wide association studies have identified ~50 loci in the human genome associated with heart attacks. However, neither the genetic variant responsible for the increased risk nor the cell type in which it functions has been identified. We are developing methods to identify which locus-linked genes are functional in platelets and the effect the common variants have on their expression and function.

  • Arterial hypertension is associated with thrombotic events due to platelet activation. The exact mechanism by which hypertensive platelets become more sensitive to agonist stimulation is unknown. We have collected platelet miRNA from healthy and pre-hypertensive subjects and found association between miRNA level and blood pressure.  We will use this data to identify differentially expressed genes responsible for the increased function in platelets.


Most Recent Peer-Reviewed Publications

  1. Common variants in the human platelet PAR4 thrombin receptor alter platelet function and differ by race
  2. MicroRNA expression differences in human hematopoietic cell lineages enable regulated transgene expression
  3. Human platelet microRNA-mRNA networks associated with age and gender revealed by integrated plateletomics
  4. The human platelet: Strong transcriptome correlations among individuals associate weakly with the platelet proteome
  5. Mechanism of race-dependent platelet activation through the protease-activated receptor-4 and Gq signaling axis
  6. Racial differences in human platelet PAR4 reactivity reflect expression of PCTP and miR-376c
  7. MicroRNAs in platelet production and activation
  8. The complex transcriptional landscape of the anucleate human platelet
  9. Small RNAs as potential platelet therapeutics
  10. Human genome-wide association and mouse knockout approaches identify platelet supervillin as an inhibitor of thrombus formation under shear stress
  11. Human genome-wide association and mouse knockout approaches identify platelet supervillin as an inhibitor of thrombus formation under shear stress
  12. High-throughput screening uncovers a compound that activates latent HIV-1 and acts cooperatively with a histone deacetylase (HDAC) inhibitor
  13. MicroRNAs in platelet production and activation
  14. Platelet microRNA-mRNA coexpression profiles correlate with platelet reactivity
  15. Short communication: Activation of latent HIV type 1 gene expression by suberoylanilide hydroxamic acid (SAHA), an HDAC inhibitor approved for use to treat cutaneous T cell lymphoma
  16. The SCAN domain family of zinc finger transcription factors
  17. Chromatin Modification and the Endothelial-specific activation of the E-selectin gene
  18. Nuclear Factor κB-Dependent Activation of the Antiapoptotic bfl-1 Gene by the Epstein-Barr Virus Latent Membrane Protein 1 and Activated CD40 Receptor
  19. NF-κB-dependent assembly of an enhanceosome-like complex on the promoter region of apoptosis inhibitor Bfl-1/A1
  20. Mechanisms of constitutive NF-κB activation in human prostate cancer cells