Philadelphia University + Thomas Jefferson University

Kaji, Hideko

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Hideko Kaji, PhD

Contact Dr. Kaji

1020 Locust Street
Room 456A JAH
Philadelphia, PA 19107

(215) 503-6547
(215) 923-7343 fax

Medical School

PhD, Purdue University

Fellowship

Recipient of the Special Fellowship from the Japanese Government, 1952-1954
Recipient of Association of American College Women Club Fellowship, 1954
Recipient of University of Nebraska Foundation Fellowship, 1954-1956

Recipient of Purdue Foundation Fellowship, 1956 -1958
Recipient of Eli Lilly Fellowship, 1958 -1959
Recipient of Senior NIH Fellowship, 1972 -1973
Recipient of Max Planck Gesellschaft Foundation Fellow, 1972 -1974
Fellow of the American Association for the Advancement of Science, 2011

Board Memberships

Elected Board Member, Scientific Counselor, National Institutes of Health, 1985 -1987

Expertise and Research Interests

Translation (protein synthesis) from aminoacyl tRNA consists of four consecutive steps; initiation, elongation, termination, and recycling of the machinery of the protein synthesis for the next round of translation. We discovered the fourth step. We are working on the this step in prokaryotes and eukaryotes. We discovered that a protein called ribosome recycling factor (RRF) is necessary for this step in prokaryotes. This protein is essential for all organisms except for Archaea. In collaboration with other laboratories, we determined the structure of RRF and the ribosomal binding site of RRF. RRF moves in the inter-subunits space of the ribosome during its function like tRNA does. We have recently discovered RRF functions to rescue stalled ribosomes with the help of termination factors RFs in the absence of the termination codon. This is being submitted.
In the eukaryotic cytoplasm, there is no equivalent of RRF for recycling ribosomes. In search for the recycling factor, we discovered recently that eEF3 and ATP functions to disassemble the post-termination complex. The P-site bound peptidyl tRNA is hydrolyzed by eRF1 and 3 to form post-termination complexes. For convenience, we can use puromycin, an antibiotic which reacts with peptidyl tRNA on the P-site to form peptidyl puromycin. Since eEF3 is limited to yeast, the system is a good target of possible anti-fungal agent. Since eEF3 is absent in higher eukaryotes like humans, there must be other protein factor responsible ribosome recycling in the cytoplasm. We are going to find out this protein. These studies are basis of fundamental biology, protein synthesis. The factor we are after may play important role in tumor formation, aging, and development.

Other Expertise

Laboratory Staff
Postdoctoral fellows:
1) Dr. Nobuhiro Iwakura, Ph.D. He received his Ph.D in microbiology at Niigata School of Medicine, Japan under the guidance of Professor Tatsuo Yamamoto in 2005. He joined our laboratory right after his Ph.D and has been with us since. He works on prokaryotic ribosome recycling system. He has made important contributions and they are listed in the list of academic report of CV of Hideko Kaji.
2) Dr. Shinya Kurata, Ph.D He received his Ph.D in biochemistry and molecular biology at School of Engineering , Tokyo University, Japan under the guidance of Professor Tsutomu Suzuki in 2007. He joined our laboratory right after his Ph.D and has been with us since. He discovered that ribosome recycling in yeast is catalyzed by elongation factor 3 and ATP. This seminal discovery was reported in Proc. Nat. Acad. Sci (June 2010). He is continuing studies on the eukaryotic ribosome recycling.

Medical Students:
1) Andrew Baron; He is a second year Jefferson Medical College student and works as a work study student. His work is related to both eukaryotic and prokaryotic ribosome recycling.
2) Tomoya Inukai: He is a fourth year Medical Student of Niigata School of Medicine (the Japanese Medical School takes students from high school and educate them for total of six years). He is a part time student who works on eukaryotic ribosome recycling system together with Dr. Shinya Kurata.
3) Sohei Abe: He is a fourth year Medical Student of Niigata School of Medicine. He is a part time student who works on prokaryotic ribosome recycling system in prokaryotes. He collaborates with Dr. Nobuhiro Iwakura.

Keywords

Biochemistry

Languages

English, German, Japanese

Publications

Most Recent Peer-Reviewed Publications

  1. The kinetic mechanism of bacterial ribosome recycling
  2. Chemical and structural characterization of a model Post-Termination Complex (PoTC) for the ribosome recycling reaction: Evidence for the release of the mRNA by RRF and EF-G
  3. Recollection of how we came across the protein modifi cation with amino acids by Aminoacyl tRNA-protein transferase
  4. Correlated measurement of endogenous ATE1 activity on native acceptor proteins in tissues and cultured cells to detect cellular aging
  5. Possible steps of complete disassembly of post-termination complex by yeast eEF3 deduced from inhibition by translocation inhibitors
  6. Global cellular regulation including cardiac function by post-translational protein arginylation
  7. Protein synthesis factors (RF1, RF2, RF3, RRF, and tmRNA) and peptidyl-tRNA hydrolase rescue stalled ribosomes at sense codons
  8. Structural insights into initial and intermediate steps of the ribosome-recycling process
  9. Protein modification by arginylation
  10. Ribosome recycling step in yeast cytoplasmic protein synthesis is catalyzed by eEF3 and ATP
  11. The role of GTP in transient splitting of 70S ribosomes by RRF (ribosome recycling factor) and EF-G (elongation factor G)
  12. Structural Insights into Ribosome Recycling Factor Interactions with the 70S Ribosome
  13. Erratum: Novel activity of eukaryotic translocase, eEF2: Dissociation of the 80S ribosome into subunits with ATP but not with GTP (Nucleic Acids Research (2007) vol. 35 (4597-4607))
  14. Structural basis for aminoglycoside inhibition of bacterial ribosome recycling
  15. Progression of the Ribosome Recycling Factor through the Ribosome Dissociates the Two Ribosomal Subunits
  16. Novel activity of eukaryotic translocase, eEF2: Dissociation of the 80S ribosome into subunits with ATP but not with GTP
  17. Ribosome recycling: An essential process of protein synthesis
  18. Inhibition of antiassociation activity of translation initiation factor 3 by paromomycin
  19. The ribosome-recycling step: Consensus or controversy?
  20. The role of ribosome recycling factor in dissociation of 70S ribosomes into subunits