Tyagi Lab research revolves around HIV and drugs of abuse focusing mainly transcriptional and epigenetic aspects. Our main research areas of interest are:
Mudit Tyagi, PhD, MSc, PGDBT
Associate Professor of Medicine
Contact Information
Associate Professor of Medicine
Research & Clinical Interests
Education
PhD, Molecular Virology, International Center for Genetic Engineering and Biotechnology (ICGEB), , Trieste, Italy
MSc, Biochemistry, Indian Veterinary Research Institute (IVRI), India
Post-graduate Diploma, Biochemical Technology, Delhi University, India
BSc (Honors), Biochemistry, Delhi University, Delhi, India
Publications
- HIV Infection, Neurotoxicity, Inflammation, Premature Aging, and Therapeutic Challenges to PLWH: An Overview
- Protocol for nucleo-cytoplasmic fractionation of mammalian cells to study protein translocation via immunoblotting
- Notch3 deletion regulates HIV-1 gene expression and systemic inflammation to ameliorate chronic kidney disease
- Cocaine-Induced DNA-Dependent Protein Kinase Relieves RNAP II Pausing by Promoting TRIM28 Phosphorylation and RNAP II Hyperphosphorylation to Enhance HIV Transcription
- An overview of the mechanisms of HIV-1 infection, latency, pathogenesis, and eradication strategies from the CNS
Research & Clinical Interests
The Tyagi Lab research revolves around HIV and drugs of abuse focusing mainly transcriptional and epigenetic aspects.
Dr. Tyagi received his PhD in the field of Molecular Biology of HIV from International center for Genetic Engineering and Biotechnology (ICGEB), Trieste, Italy.
His main research areas of interest are:
- Define the underlying molecular mechanisms that regulate HIV transcription and latency, and how can those mechanisms be used for therapeutic benefits.
- Characterize the molecular mechanisms that different drugs of abuse modulate to enhance HIV replication and neurocognitive complications.
The main techniques we utilize are RNA-Seq, ChIP-Seq, ATAC-Seq Western blotting, cloning, flowcytometry, mutagenesis, human cell culture (primary and cell lines), protein expression and purification, enzymatic assays, light and fluorescent microscopy, confocal microscopy, transcriptomic and proteomic analyses, transcription and signal transduction assays, besides other usual molecular biology lab assays.