Philadelphia University + Thomas Jefferson University

Risbud, Makarand V.

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Makarand V. Risbud, PhD

Makarand V. Risbud, PhD

Contact Dr. Risbud

1015 Walnut Street
Suite 501, Curtis Bldg.
Philadelphia, PA 19107

(215)-955-1063

Education

PhD, National Center for Cell Science, India - 2001
M.Tech., Indian Institute of Technology (IIT): Bombay - 1999
MSc, Biochemistry, University of Pune - 1997

Fellowship

Post Doc Fellowship, Harvard University, Boston, 2001-2002

University Appointments

James J. Maguire Jr. Professor of Spine Research, Department of Orthopaedic Surgery
Co-Director, Cell Biology & Regenerative Medicine Graduate Program

Expertise & Research Interests

Major research focus of my lab is to study the mechanisms by which nucleus pulposus cells of the intervertebral disc adapt to avascular (hypoxic) and osmotically compromising environment. In this regard, we are studying role of transciption factors HIFs and TonEBP and signaling events that control their function. Another major research interest is to device tissue-enginering based strategies for intervertebral disc regeneration. We are testing hypothesis that adult mesenchymal stem cells (MSC) transplanted into the disc will assume nucleus pulposus-like phenotype and achieve functional restoration of degenerate disc. Our recent work shows that MSC differentiate into nucleus pulposus-like cells when cultured under conditions similar to that exist in the disc in vivo. A whole disc organ culture is also under investigation to understand aspects of disc cell function in an ex vivo setting.

Other Expertise

Cell-Scaffold intereactions at molecular level and chitosan based scaffolds

Industrial Relevance

My reaserch has a strong translational component. Regeneration of degenerate intervertberal disc using adult-mesenchymal stem cells could be a clinical therapy in future and is of immense interest to many biotech and health science companies.

Keywords

Intervertebral Disc; Mesenchymal Stem Cells; Nucleus Pulposus Cells; Hypoxia; HIF-1, hypertonicity, TonEBP, Cell signaling; Tissue Engineering, Scaffolds

Languages

English, Hindi, Marathi

Publications

Most Recent Peer-Reviewed Publications

  1. Expression of Carbonic Anhydrase III, a Nucleus Pulposus Phenotypic Marker, is Hypoxia-responsive and Confers Protection from Oxidative Stress-induced Cell Death
  2. Bicarbonate Recycling by HIF-1–Dependent Carbonic Anhydrase Isoforms 9 and 12 Is Critical in Maintaining Intracellular pH and Viability of Nucleus Pulposus Cells
  3. Glycosaminoglycan synthesis in the nucleus pulposus: Dysregulation and the pathogenesis of disc degeneration
  4. A novel mouse model of intervertebral disc degeneration shows altered cell fate and matrix homeostasis
  5. COX-2 expression mediated by calcium-TonEBP signaling axis under hyperosmotic conditions serves osmoprotective function in nucleus pulposus cells
  6. Lack of evidence for involvement of TonEBP and hyperosmotic stimulus in induction of autophagy in the nucleus pulposus
  7. PHD3 is a transcriptional coactivator of HIF-1a in nucleus pulposus cells independent of the PKM2-JMJD5 axis
  8. New horizons in spine research: Intervertebral disc repair and regeneration
  9. TNF-α promotes nuclear enrichment of the transcription factor TonEBP/NFAT5 to selectively control inflammatory but not osmoregulatory responses in nucleus pulposus cells
  10. RNA sequencing reveals a role of TonEBP transcription factor in regulation of pro-inflammatory genes in response to hyperosmolarity in healthy nucleus pulposus cells a homeostatic response?
  11. Hypoxia promotes noncanonical autophagy in nucleus pulposus cells independent of MTOR and HIF1A signaling
  12. New Horizons in Spine Research: Disc biology, spine biomechanics and pathomechanisms of back pain
  13. Molecular mechanisms of biological aging in intervertebral discs
  14. N-cadherin is key to expression of the nucleus pulposus cell phenotype under selective substrate culture conditions
  15. Class i and IIa HDACs Mediate HIF-1α Stability Through PHD2-Dependent Mechanism, while HDAC6, a Class IIb Member, Promotes HIF-1α Transcriptional Activity in Nucleus Pulposus Cells of the Intervertebral Disc
  16. Syndecan-4 in intervertebral disc and cartilage: Saint or synner?
  17. The mRNA-binding protein HuR promotes hypoxia-induced chemoresistance through posttranscriptional regulation of the proto-oncogene PIM1 in pancreatic cancer cells
  18. Circadian factors BMAL1 and RORα control HIF-1α transcriptional activity in nucleus pulposus cells: Implications in maintenance of intervertebral disc health
  19. TGFβ regulates Galectin-3 expression through canonical Smad3 signaling pathway in nucleus pulposus cells: Implications in intervertebral disc degeneration
  20. Matrix vesicles: Are they anchored exosomes?